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Updated: Aug 19, 2026

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
Published on: October 28, 2022
Preparation and study of human eyes obtained postmortem with the Miyake posterior photographic technique
D J Apple1, E S Lim, R C Morgan
1Department of Ophthalmology, Storm Eye Institute, Medical University of South Carolina, Charleston 29425.
Abstract:
A modification of the original Miyake technique of posterior photography of human eyes obtained postmortem is presented. A detailed description of globe preparation and equipment is described. Use of modern technology including a high magnification microscope system and video camera permits dynamic or realtime observations of tissue effects of various surgical procedures such as the cataract operation. This technique provides both a research and educational tool that can be used by ophthalmologists to improve the quality of surgery. Most important, it is an instructional tool that should reduce the "learning curve" that is inherent when modifying one's surgical technique.

