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On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis
Published on: May 31, 2020
On-line solid phase extraction-liquid chromatography, with emphasis on modern bioanalysis and miniaturized systems
Magnus Rogeberg1, Helle Malerod, Hanne Roberg-Larsen
1Department of Chemistry, University of Oslo, Post Box 1033, Blindern, N-0315 Oslo, Norway.
Journal of Pharmaceutical and Biomedical Analysis
|June 11, 2013
Summary
Online solid phase extraction-liquid chromatography (SPE-LC) offers automated, sensitive bioanalysis, especially in miniaturized systems. Exploring diverse SPE-LC combinations enhances analytical capabilities for complex biological samples.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Chromatography
Background:
- Online solid phase extraction-liquid chromatography (SPE-LC) is crucial for automated, sensitive bioanalysis.
- Miniaturized or nano LC systems are ideal for high sensitivity and limited sample analysis.
- Traditional SPE-LC columns use reversed-phase materials, but diverse phases are emerging.
Purpose of the Study:
- To review the expanding diversity of SPE-LC combinations for bioanalytical challenges.
- To discuss challenges and solutions in combining different SPE-LC phases.
- To highlight the advantages of non-particulate columns in miniaturized SPE-LC systems.
Main Methods:
- Review of current online SPE-LC combinations and materials (PGC, HILIC, RAM, immunoaffinity, metal oxide).
- Analysis of issues arising from phase incompatibilities in SPE-LC.
- Evaluation of miniaturized systems with narrow, non-particulate columns (monolithic, PLOT).
Main Results:
- Diverse SPE-LC phases (PGC, HILIC, RAM, etc.) are employed for various applications like metabolomics, glycomics, and proteomics.
- Solvent incompatibilities can lead to low recovery and poor resolution in SPE-LC.
- Non-particulate columns in narrow SPE-LC systems (10-20 μm ID) reduce extra-column volumes and enhance sensitivity.
Conclusions:
- Online SPE-LC technology is versatile, with diverse phases and miniaturized systems offering advanced bioanalytical solutions.
- Addressing solvent compatibility and utilizing non-particulate columns can improve SPE-LC performance.
- Configurable SPE-LC systems with optimized columns and hardware enhance robustness and analytical capabilities.
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