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Updated: May 10, 2026
11:49
Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
Published on: May 2, 2013
Cytokine gene polymorphisms in kidney transplantation.
1Laboratory of Research in Immunology of Renal Transplantation and Immunopathology (LR03SP01), University Tunis EI Manar, Charles Nicolle Hospital, Tunis, Tunisia. dhaouaditarak@yahoo.fr
Transplantation Proceedings
|June 11, 2013
Summary
Cytokine gene polymorphisms, including tumor necrosis factor-alpha and transforming growth factor-beta1, do not predict kidney transplant rejection alone. However, specific haplotypes are linked to acute rejection episodes and poorer graft survival.
Area of Science:
- Immunogenetics
- Transplantation immunology
- Renal transplantation
Background:
- Acute and chronic rejection are primary causes of kidney graft loss.
- Cytokine genotyping may predict rejection risk independent of HLA compatibility or anti-HLA antibodies.
Purpose of the Study:
- Investigate cytokine polymorphisms (TNF-α, TGF-β1, IL-10, IL-6, IFN-γ) in kidney transplantation.
- Examine their predictive roles for acute or chronic rejection.
Main Methods:
- Analyzed single nucleotide polymorphisms (SNPs) for TNF-α, TGF-β1, IL-10, IL-6, and IFN-γ in 231 kidney transplant recipients (KTR).
- Utilized polymerase chain reaction (PCR)-specific sequence primers (SSP) for SNP detection.
- Included KTR treated with mycophenolate mofetil (MMF+) and those without (MMF-).
Main Results:
- No single polymorphism was significantly associated with acute rejection (ARE) or chronic allograft dysfunction (CAD).
- TGF-β1 Low production showed a trend towards better graft survival (81.8% at 20 years) but was not significant (P = .2).
- In MMF- KTR without HLA-mismatches or pre-formed antibodies, the TNF-α*H/TGF-β1*H/IL-10*H haplotype was associated with significantly higher ARE (75%) compared to others (16%; P = .03).
Conclusions:
- The TGF-β1 High secretion profile may offer kidney graft protection.
- The TNF-α*H/TGF-β1*H/IL-10*H haplotype is linked to increased ARE risk and diminished graft survival.
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