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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: May 10, 2026

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers
12:37

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers

Published on: September 4, 2015

Magnetic separations in biotechnology.

L Borlido1, A M Azevedo, A C A Roque

  • 1Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Department of Bioengineering, Instituto Superior Técnico, Technical University of Lisbon, Av. Rovisco Pais, Lisbon 1049-001, Portugal.

Biotechnology Advances
|June 11, 2013
PubMed
Summary

Magnetic adsorbents offer versatile, fast, and gentle purification of biologicals like cells and proteins. This review covers their synthesis, tailoring, and applications in biotechnology separations.

Keywords:
AutomationMagnetic particlesMagnetic separationsPurificationSynthesis

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Area of Science:

  • Biotechnology
  • Materials Science
  • Chemical Synthesis

Background:

  • Magnetic separations are highly versatile for purifying biological molecules from crude samples.
  • This technique offers advantages in speed, gentleness, scalability, and automation compared to alternatives.
  • Magnetic adsorbents are central to this process, requiring complex synthesis across scientific disciplines.

Purpose of the Study:

  • To review the synthesis and tailoring of magnetic adsorbents for bioseparation.
  • To discuss the application of these tailored magnetic adsorbents in biotechnology.

Main Methods:

  • Review of scientific literature on magnetic adsorbent synthesis.
  • Analysis of tailoring strategies for specific biomolecule targets.
  • Examination of case studies on the application of magnetic adsorbents in bioseparation.

Main Results:

  • Magnetic adsorbents can be synthesized using diverse methods.
  • Tailoring enables specific capture of cells, viruses, proteins, and nucleic acids.
  • Successful applications demonstrated across various bioseparation challenges.

Conclusions:

  • The synthesis and tailoring of magnetic adsorbents are critical for advanced bioseparation.
  • These materials provide efficient and scalable solutions for biotechnological purification processes.