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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: May 10, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Changes in splicing factor expression are associated with advancing age in man.

Alice C Holly1, David Melzer, Luke C Pilling

  • 1Institute of Biomedical and Clinical Sciences, University of Exeter Medical School, University of Exeter, Exeter EX1 2LU, UK.

Mechanisms of Ageing and Development
|June 11, 2013
PubMed
Summary
This summary is machine-generated.

Human aging alters splicing factor expression and alternative splicing. The DNA damage response protein ATM may inhibit splicing factor expression, impacting cellular adaptability.

Keywords:
ATMAgeingAtaxia Telangiectasia Mutated geneHeterogeneous nuclear ribonucleoproteinsHumanLMNALamin A/C geneRT-PCRReverse Transcription PCRSR splicing factorSRSFSplicingTLDATaqMan Low Density ArrayhnRNPqRT-PCRquantitative real-time PCRsiRNAsmall interfering RNA

More Related Videos

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Related Experiment Videos

Last Updated: May 10, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Area of Science:

  • Molecular Biology
  • Genetics
  • Aging Research

Background:

  • Human aging is characterized by reduced cellular plasticity.
  • Age-related changes in alternative splicing are linked to altered splicing factor expression.
  • Understanding these molecular changes is crucial for aging research.

Purpose of the Study:

  • To investigate age-related changes in mRNA expression of key splicing factors.
  • To determine the role of Ataxia Telangiectasia Mutated (ATM) in regulating splicing factor expression.
  • To explore the impact of ATM on alternative splicing in aging cells.

Main Methods:

  • Microarray analysis of blood from two human populations.
  • Quantitative real-time PCR (qRT-PCR) in senescent fibroblasts and endothelial cells.
  • Small interfering RNA (siRNA) analysis to investigate gene regulation.

Main Results:

  • Approximately one-third of splicing factors showed age-related transcript expression changes.
  • ATM transcript expression correlated with splicing factor expression in human data.
  • Senescent cells exhibited altered splicing factor expression and alternative splicing patterns.

Conclusions:

  • Splicing factor expression and isoform ratios change with age, both in vivo and in vitro.
  • ATM may play an inhibitory role in the expression of certain splicing factors.
  • ATM, a DNA damage response protein, is identified as a key regulator of the human splicing machinery.