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Related Experiment Video

Updated: May 10, 2026

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

PPP3CC feedback regulates IP3-Ca2+ pathway through preventing ITPKC degradation.

Pu Li¹, Peng Zhang, Ying Lin

¹State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.

Frontiers in Bioscience (Landmark Edition)

|June 11, 2013

Summary

Researchers discovered PPP3CC interacts with ITPKC, a gene linked to Kawasaki disease. PPP3CC stabilizes ITPKC levels, impacting the IP3-Ca2+ signaling pathway crucial for immune responses.

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Published on: January 7, 2013

Area of Science:

Immunology
Molecular Biology
Cell Signaling

Background:

Inositol trisphosphate kinase C (ITPKC) is a susceptibility gene for Kawasaki disease.
ITPKC regulates intracellular calcium (Ca2+) levels and calcineurin/NFAT signaling.
Understanding ITPKC regulation is crucial for Kawasaki disease research.

Purpose of the Study:

To identify novel proteins interacting with ITPKC.
To elucidate the functional role of ITPKC-interacting proteins in cellular signaling.
To investigate the impact of these interactions on the IP3-Ca2+ pathway.

Main Methods:

Yeast two-hybrid screening to identify interacting proteins.
GST pull-down and co-immunoprecipitation assays for interaction validation.
Fluorescent microscopy for protein co-localization studies.
Western blotting to assess protein levels and phosphorylation status.

Main Results:

A novel ITPKC-interacting protein, PPP3CC, was identified and validated.
PPP3CC and ITPKC co-localize in the cell cytoplasm.
PPP3CC enhances ITPKC protein stability by inhibiting its phosphorylation and subsequent degradation.
PPP3CC levels negatively correlate with cellular inositol trisphosphate (IP3) levels.

Conclusions:

PPP3CC is a novel regulator of ITPKC stability and function.
PPP3CC plays a significant role in modulating the IP3-Ca2+ signaling pathway.
These findings provide new insights into the molecular mechanisms underlying Kawasaki disease pathogenesis.