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Updated: May 10, 2026

Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
Published on: March 16, 2017
Use of the Ussing chamber technique to study nutrient transport by epithelial tissues
Liuqin He1, Yulong Yin, Tiejun Li
1Chinese Academy of Sciences, Institute of Subtropical Agriculture, Research Center for Healthy Breeding Livestock and Poultry, Changsha, Hunan, China.
Abstract:
The Ussing chamber provides a physiologically relevant system for measuring the transport of ions, nutrients, and drugs across various epithelial tissues. This article outlines the design, structure, principle, and operation of the Ussing chamber, its application in the field of gastrointestinal barrier function and nutrient transport research, as well as its advantages and limitations. This review serves as a practical guide for investigators who are new to the Ussing chamber and should help researchers better understand this valuable method for measuring the transport of electrolytes, organic nutrients, water, and drugs across the small intestine, placenta, and other epithelial tissues.
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