Related Experiment Video
Updated: May 10, 2026
Triggering Cell Stress and Death Using Conventional UV Laser Confocal Microscopy
Published on: February 3, 2017
A method for selective ablation of neurons in C. elegans using the phototoxic fluorescent protein, KillerRed
Junya Kobayashi1, Hisashi Shidara, Yuma Morisawa
1Center for Biosciences and Informatics, School of Fundamental Sciences and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama, Kanagawa, Japan.
Abstract:
Specific neuron ablation with laser microbeam has been used in behavioral analysis of Caenorhabditis elegans. However, this method is hard to acquire many ablated worms, and is unable to compare behavioral changes just before and after ablation. Here, we developed an ablation method by using genetically encoded photosensitizer protein, KillerRed, which produces reactive oxygen species by green light irradiation. Ablation of AWA sensory neurons abolished the chemotaxis to AWA specific sensitive attractant, diacetyl, and no functional effect on the other sensory neuron, AWC, which senses benzaldehyde. This ablation method can be useful for analyzing neural in situ.
