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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Related Experiment Video

Updated: May 10, 2026

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

Noise models and cryo-EM drift correction with a direct-electron camera.

H Shigematsu1, F J Sigworth

  • 1Department of Cellular and Molecular Physiology, Yale University, 333 Cedar Street, New Haven, CT 06520, United States. hideki.shigematsu@yale.edu

Ultramicroscopy
|June 11, 2013
PubMed
Summary

Specimen holder drift in cryo-electron microscopy (cryo-EM) images can be corrected. Analyzing frame sequences from high-speed cameras allows for tracking and compensating drift, improving image quality.

Keywords:
CMOSComplementary metal-oxide-semiconductorCross-correlation functionCryo-EMElectron cryomicroscopyTransmission electron microscopy

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Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
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Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Published on: July 1, 2022

Related Experiment Videos

Last Updated: May 10, 2026

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon
04:52

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Published on: July 1, 2022

Area of Science:

  • Microscopy
  • Structural Biology
  • Image Analysis

Background:

  • Specimen holder drift causes blurring in cryo-electron microscopy (cryo-EM) images.
  • High frame rate cameras acquire low-dose exposures as image sequences.

Purpose of the Study:

  • To track and compensate for specimen holder drift in cryo-EM images.
  • To address challenges posed by hot-pixel noise in image analysis.

Main Methods:

  • Analyzing frame sequences from DE-12 cameras.
  • Developing a noise model to reduce hot-pixel noise effects.
  • Deriving a filter function for optimized cross-correlation tracking.

Main Results:

  • Successfully modeled and reduced hot-pixel noise.
  • Demonstrated effective tracking of specimen drift in cryo-EM images.
  • Improved image quality by compensating for drift.

Conclusions:

  • Frame sequence analysis is a viable method for drift correction in cryo-EM.
  • The developed noise reduction and tracking methods enhance image clarity and data quality.