Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cholesterol depletion inhibits BDNF-dependent spike timing-dependent plasticity at thalamo-amygdala synapses.

Frontiers in cellular neuroscience·2026
Same author

Association between S-ketamine induced changes in glutamate levels in the pregenual anterior cingulate cortex and plasma brain-derived neurotrophic factor in healthy subjects.

Frontiers in psychiatry·2025
Same author

Circadian rhythm of brain-derived neurotrophic factor in serum and plasma.

Experimental physiology·2024
Same author

Controlled Hypoxia Acutely Prevents Physical Inactivity-Induced Peripheral BDNF Decline.

International journal of molecular sciences·2024
Same author

Distinct GABAergic modulation of timing-dependent LTP in CA1 pyramidal neurons along the longitudinal axis of the mouse hippocampus.

iScience·2024
Same author

Brain-Derived neurotrophic factor and inflammatory biomarkers are unaffected by acute and chronic intermittent hypoxic-hyperoxic exposure in geriatric patients: a randomized controlled trial.

Annals of medicine·2024

Related Experiment Video

Updated: May 10, 2026

One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Single-cell juxtacellular transfection and recording technique.

Julia Daniel1, Hans Reiner Polder, Volkmar Lessmann

  • 1Institute of Physiology, Medical Faculty, Otto-von-Guericke-University, Magdeburg, Germany.

Pflugers Archiv : European Journal of Physiology
|June 11, 2013
PubMed
Summary

Researchers developed a novel single-cell transfection method for neurons. This technique allows precise genetic modification and subsequent electrophysiological analysis without disrupting cellular networks.

More Related Videos

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
12:59

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Published on: January 19, 2011

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
10:29

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

Published on: June 3, 2011

Related Experiment Videos

Last Updated: May 10, 2026

One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
12:59

Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells

Published on: January 19, 2011

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex
10:29

Whole Cell Recording from an Organotypic Slice Preparation of Neocortex

Published on: June 3, 2011

Area of Science:

  • Neuroscience
  • Cell Biology
  • Molecular Biology

Background:

  • Studying protein function in living cells often involves bulk methods.
  • Subtle mechanisms require fine-tuned, single-cell level analysis without systemic imbalance.

Purpose of the Study:

  • To develop and validate a single-cell transfection method for neurons.
  • To enable analysis of genetic modification consequences at the single-cell level.

Main Methods:

  • Developed a juxtacellular recording and transfection method in loose patch configuration.
  • Utilized patch clamp amplifiers for loose seal recordings and electroporation for transfection.
  • Transfected CA1 pyramidal neurons with siRNA and GFP plasmids.

Main Results:

  • Successfully identified and transfected individual CA1 pyramidal neurons.
  • Electrophysiological properties (action potential firing, synaptic transmission) remained comparable to untransfected cells post-transfection.
  • Method allows for subsequent analysis of morphological and electrophysiological parameters.

Conclusions:

  • The developed method enables precise single-cell genetic modification in neurons.
  • This technique preserves neuronal function, allowing detailed post-modification analysis.
  • Facilitates understanding of protein function at the single-cell level.