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Updated: May 10, 2026

Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
Published on: December 15, 2015
New advances in countercurrent chromatography and centrifugal partition chromatography: focus on coupling strategy
Thomas Michel1, Emilie Destandau, Claire Elfakir
1Université d'Orléans-CNRS, Institut de Chimie Organique et Analytique UMR 7311, BP 6759 rue de Chartres, 45067, Orléans cedex 2, France.
Abstract:
Countercurrent chromatography (CCC) is an attractive separation method because the analytes are partitioned between two immiscible liquid phases avoiding problems related to solid stationary phase. In recent years, this technique has made great progress in separation power and detection potential. This review describes coupling strategies involving high speed CCC (HSCCC) or centrifugal partition chromatography (CPC). It includes on-line extraction-isolation, hyphenation with mass spectrometry (MS) and nuclear magnetic resonance (NMR) detectors, multidimensional CCC (MDCCC), two-dimensional CCC (2D-CCC), on-line coupling with liquid chromatography (LC), and biological tests, and innovative off-line developments. The basic principles of each method are presented and applications are summarized.
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