Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
Atomic Spectroscopy: Effects of Temperature01:27

Atomic Spectroscopy: Effects of Temperature

Atomization, converting samples into gas-phase atoms and ions, is essential for atomic spectroscopy. The flame temperature required for atomization affects the efficiency of the atomic spectroscopic methods by increasing the atomization efficiency and the relative population of the excited and ground states.
At thermal equilibrium, the relative populations of excited and ground state atoms can be estimated using the Maxwell–Boltzmann distribution. For example, an increase in temperature from...
Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals01:17

Electron Paramagnetic Resonance (EPR) Spectroscopy: Organic Radicals

Ideally, an unpaired electron shows a single peak in the EPR spectrum due to the transition between the two spin energy states. However, coupling interactions can occur between the spins of the unpaired electron and any neighboring spin-active nuclei. This hyperfine coupling results in hyperfine splitting, where the EPR signal is split into multiplets. The signals split into 2nI + 1 peaks, where n is the number of equivalent nuclei and I is the nuclear spin. These splitting patterns provide...
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
Nuclear Overhauser Enhancement (NOE)01:06

Nuclear Overhauser Enhancement (NOE)

Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective Alcohol Oxidation by a Copper TEMPO Catalyst: Mechanistic Insights by Simultaneously Coupled Operando EPR/UV-Vis/ATR-IR Spectroscopy.

Angewandte Chemie (International ed. in English)·2015
Same author

Physical and chemical response of FeCl3/FeCl4(-) spin probes on the functionalizing of ionic liquids.

The journal of physical chemistry. A·2013
Same author

Molecular photoswitches based on spiro-acridans.

Chemical communications (Cambridge, England)·2013
Same author

Tuning the electronic and spin complexity in organic-inorganic molecular hybrid compounds.

Chemistry (Weinheim an der Bergstrasse, Germany)·2012
Same author

Spin exchange of nitroxyl radicals in H2O and D2O.

The journal of physical chemistry. A·2011
Same author

The microwave cavity perturbation technique for contact-free and in situ electrical conductivity measurements in catalysis and materials science.

Physical chemistry chemical physics : PCCP·2011

Related Experiment Video

Updated: May 10, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
08:17

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Published on: July 9, 2021

Isotope effects in ESR spectroscopy.

Reinhard Stößer1, Werner Herrmann

  • 1Institute of Chemistry, Humboldt University of Berlin, Brook-Taylor-Str. 2, Berlin 12489, Germany.

Molecules (Basel, Switzerland)
|June 11, 2013
PubMed
Summary

Electron Spin Resonance (ESR) spectroscopy can reveal isotope effects by considering time and mass dependencies. Incorporating isotope effects is essential for a comprehensive understanding of ESR spectroscopy and paramagnetic systems.

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Quantum Mechanics

Background:

  • Electron Spin Resonance (ESR) spectroscopy is a powerful tool for studying paramagnetic species.
  • Current models often overlook time and mass dependencies, limiting the interpretation of ESR spectra.
  • Isotope effects play a crucial role in understanding molecular dynamics and structure.

Purpose of the Study:

  • To elucidate the relationship between ESR spectroscopy and isotope effects.
  • To develop generalized models for isotope effects in ESR.
  • To demonstrate the indispensable role of isotope effects in ESR spectroscopy.

Main Methods:

  • General description of ESR spectroscopy and interpretation models.
  • Development of generalized isotope effect characteristics.

More Related Videos

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
09:40

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

Related Experiment Videos

Last Updated: May 10, 2026

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA
08:17

Practical Aspects of Sample Preparation and Setup of 1H R1ρ Relaxation Dispersion Experiments of RNA

Published on: July 9, 2021

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
09:40

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping

Published on: August 26, 2010

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
08:01

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo

Published on: September 26, 2016

  • Application of quantum mechanical principles to couple electron spins with molecular degrees of freedom.
  • Formalization of ESR parameters and models to include time and mass dependencies.

    • Main Results:

    • ESR models are presented that incorporate time and mass dependencies, reflecting specific isotope effects.
    • Relationships are established between ESR spectral effects and phenomena like spin relaxation, spin exchange, magnetic isotope effects, Jahn-Teller effects, and zero-point vibrations.
    • Examples illustrate the influence of isotopes and the information accessible through ESR spectroscopy.

    Conclusions:

    • Isotope effects provide crucial insights into paramagnetic systems and their dynamics.
    • Generalized models enhance the interpretation of ESR spectra by including isotope effects.
    • The study highlights the indispensable nature of isotope effects in advancing ESR spectroscopy.