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Protein Folding01:22

Protein Folding

Overview

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Related Experiment Video

Updated: May 10, 2026

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
09:15

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Published on: November 21, 2017

DNA triplex folding: moderate versus high salt conditions.

Nam-Kyung Lee1, Albert Johner, Il-Buem Lee

  • 1Department of Physics, Sejong University, 143-743, Seoul, South Korea. lee@sejong.ac.kr

The European Physical Journal. E, Soft Matter
|June 11, 2013
PubMed
Summary

Researchers studied DNA triplex folding and unfolding kinetics using single-molecule FRET. Folding time depends on salt, while unfolding is constant, revealing salt-dependent folding pathways for DNA triplex formation.

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Related Experiment Videos

Last Updated: May 10, 2026

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
09:15

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Published on: November 21, 2017

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

Area of Science:

  • Biophysics
  • Molecular Biology
  • Genetics

Background:

  • Specific DNA sequences can form triple-helical structures (triplex DNA) with a separate strand.
  • Understanding DNA triplex formation is crucial for various biological processes and therapeutic applications.

Purpose of the Study:

  • To investigate the folding and unfolding kinetics of DNA triplex formation under neutral pH conditions.
  • To determine the influence of salt concentration on DNA triplex folding and unfolding dynamics.
  • To explore the relationship between salt-dependent DNA stiffness and triplex folding pathways.

Main Methods:

  • Single-molecule Förster Resonance Energy Transfer (smFRET) experiments were employed.
  • DNA strands were designed to fold into a triplex structure.
  • Folding and unfolding time distributions were measured at varying salt concentrations (10 mM and 100 mM).

Main Results:

  • Average folding and unfolding times for DNA triplexes are approximately 1 second.
  • Folding time shows moderate sensitivity to salt concentration.
  • Unfolding time remains relatively constant across different salt concentrations.
  • Heterogeneous unfolding kinetics were observed at moderate salt (10 mM) but not at high salt (100 mM).

Conclusions:

  • Salt concentration influences DNA triplex folding pathways and stability.
  • The stiffness of the single-stranded DNA donor sequence, modulated by salt, governs these salt-dependent folding behaviors.
  • The study provides insights into intramolecular triplex (H-DNA) formation under physiological conditions.