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Related Concept Videos

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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Updated: May 10, 2026

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Whole-rat conditional gene knockout via genome editing.

Andrew J Brown1, Daniel A Fisher, Evguenia Kouranova

  • 1Sage Labs, St. Louis, Missouri, USA.

Nature Methods
|June 11, 2013
PubMed
Summary

Researchers created tissue-specific knockout rats using zinc-finger nucleases (ZFNs). This efficient method enables precise gene disruption in vivo for advanced functional genomics and medical research.

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Area of Science:

  • Genetics and Genomics
  • Animal Models
  • Molecular Biology

Background:

  • Genetically modified animal models are crucial for understanding gene function and disease.
  • Temporal and spatial control over genetic modifications enhances research precision.

Purpose of the Study:

  • To develop a method for generating tissue-specific knockout rats.
  • To demonstrate the efficacy of zinc-finger nucleases (ZFNs) for creating conditional knockout models.

Main Methods:

  • Microinjection of ZFNs into fertilized rat eggs.
  • Generation of rats with floxed alleles and a Cre recombinase transgene.
  • Demonstration of Cre-dependent gene disruption in specific tissues.

Main Results:

  • Successful generation of tissue-specific knockout rats.
  • Efficient and rapid gene disruption in vivo using ZFNs.
  • Validation of the conditional knockout system.

Conclusions:

  • Pronuclear microinjection of ZFNs is an effective technique for creating conditional knockout rats.
  • This method offers a valuable tool for functional genomics and medical research.
  • The approach is potentially applicable to other species.