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Updated: May 10, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
Published on: February 27, 2020
Integrated proteomic analysis of post-translational modifications by serial enrichment
Philipp Mertins1, Jana W Qiao, Jinal Patel
1The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. pmertins@broadinstitute.org
Abstract:
We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.
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