Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Methylphenidate on Heart Rate Variability in Children and Adolescents With ADHD: A Systematic Review and Meta-Analysis.

Journal of the American Psychiatric Nurses Association·2026
Same author

Integrating transcriptomics and gene-level interpretable probabilistic Tsetlin Machine reveals elevated pancreatic cancer risk in Type 2 diabetes.

Computational biology and chemistry·2026
Same author

Effects of Exercise on Cardiometabolic Risk in Post-Bariatric Patients: An Umbrella Review of Systematic Reviews and Meta-Analyses.

Clinical obesity·2026
Same author

Reply - Letter to the editor.

Clinical nutrition ESPEN·2026
Same author

Overview of systematic reviews and meta-analysis of risperidone on cardiovascular risk: analysis of Cochrane and non-Cochrane systematic reviews including 11,949 patients.

International journal of psychiatry in clinical practice·2026
Same author

Effect of vitamin C supplementation on post-exercise recovery: A systematic review and meta-analysis of randomized double-blind placebo trials.

Clinical nutrition ESPEN·2026

Related Experiment Video

Updated: May 10, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
09:32

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

Simultaneous DNA amplification and detection using a pH-sensing semiconductor system.

Christofer Toumazou1, Leila M Shepherd, Samuel C Reed

  • 1DNA Electronics Ltd., London, UK. chris.toumazou@dnae.co.uk

Nature Methods
|June 11, 2013
PubMed
Summary
This summary is machine-generated.

This study introduces a novel semiconductor chip for real-time nucleic acid detection, directly measuring pH changes during amplification. This label-free, non-optical method enables cost-effective, portable genetic analysis.

More Related Videos

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Related Experiment Videos

Last Updated: May 10, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
09:32

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
11:40

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

Published on: November 14, 2018

Area of Science:

  • Biotechnology
  • Semiconductor Technology
  • Molecular Diagnostics

Background:

  • Traditional nucleic acid detection methods often rely on indirect, label-based measurements.
  • Existing technologies can be costly, non-portable, and require complex optical setups.
  • There is a need for direct, label-free, and real-time nucleic acid analysis platforms.

Purpose of the Study:

  • To develop an integrated system-on-chip platform for real-time nucleic acid amplification and detection.
  • To implement a novel amplification-coupled detection method utilizing pH sensing.
  • To demonstrate the platform's capability for genetic variant genotyping.

Main Methods:

  • Developed an integrated chip with ion-sensitive field-effect transistor (ISFET) sensors, temperature sensors, and control circuitry.
  • Utilized a label-free, non-optical detection strategy measuring hydrogen ion release during nucleotide incorporation.
  • Evaluated the platform with polymerase chain reaction (PCR) and isothermal amplification.
  • Applied the system to genotype single-nucleotide polymorphism (SNP) variants from human saliva samples.

Main Results:

  • Successfully demonstrated real-time, label-free detection and quantification of nucleic acid sequences.
  • Achieved direct measurement of pH signatures from native amplification chemistries.
  • Accurately genotyped single-nucleotide polymorphism (SNP) variants of the cytochrome P450 family using crude saliva.
  • Validated the platform's performance with both PCR and isothermal amplification strategies.

Conclusions:

  • The developed complementary metal-oxide semiconductor (CMOS) technology enables direct, real-time nucleic acid analysis.
  • This semiconductor-based platform offers a cost-effective, portable, and scalable solution for genetic analysis.
  • The technology has the potential to significantly advance point-of-care diagnostics and molecular testing.