Related Experiment Video
Updated: May 10, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Isolation and characterization of ethanol tolerant yeast strains
Chiranjeevi Tikka1, Hari Prasad Osuru, Navya Atluri
1Division of Animal Biotechnology, Dept. of Zoology, S.V. University, Tirupati, AP, India.
Abstract:
Yeast strains are commonly associated with sugar rich environments. Various fruit samples were selected as source for isolating yeast cells. The isolated cultures were identified at Genus level by colony morphology, biochemical characteristics and cell morphological characters. An attempt has been made to check the viability of yeast cells under different concentrations of ethanol. Ethanol tolerance of each strain was studied by allowing the yeast to grow in liquid YEPD (Yeast Extract Peptone Dextrose) medium having different concentrations of ethanol. A total of fifteen yeast strains isolated from different samples were used for the study. Seven strains of Saccharomyces cerevisiae obtained from different fruit sources were screened for ethanol tolerance. The results obtained in this study show a range of tolerance levels between 7%-12% in all the stains. Further, the cluster analysis based on 22 RAPD (Random Amplified polymorphic DNA) bands revealed polymorphisms in these seven Saccharomyces strains.
Related Concept Videos
Bioreactor Controls-III
Microbes in Beverage Production
Microbial Fermentation
Techniques for Isolation of Pure Cultures

