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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: May 10, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Managing microRNAs with vector-encoded decoy-type inhibitors.

Rasmus O Bak1, Anne Kruse Hollensen, Jacob Giehm Mikkelsen

  • 1Department of Biomedicine, Aarhus University, DK-8000 Aarhus C, Denmark.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|June 12, 2013
PubMed
Summary

MicroRNA (miRNA) inhibitors offer therapeutic potential but face delivery challenges. Vector-encoded strategies and novel RNA circles show promise for targeted, long-term miRNA suppression in research and medicine.

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Last Updated: May 10, 2026

MicroRNA-based Regulation of Picornavirus Tropism
09:05

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Published on: February 6, 2017

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
06:53

A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA

Published on: December 26, 2015

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Drug Discovery

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression, making them attractive therapeutic targets.
  • Current synthetic miRNA inhibitors face limitations like high cost, repeated dosing, and poor tissue specificity.

Purpose of the Study:

  • To review vector-encoded strategies for miRNA suppression.
  • To explore the potential of RNA circles as novel carriers for miRNA inhibitors.

Main Methods:

  • Overview of existing vector-encoded miRNA inhibitor technologies.
  • Discussion of advantages of vector-based approaches for spatiotemporal and sustained miRNA control.
  • Exploration of trans-splicing RNA circles for delivering miRNA decoys.

Main Results:

  • Vector-encoded miRNA suppression offers advantages in control and duration.
  • Naturally occurring circular RNAs, particularly those from trans-splicing, are suitable carriers for miRNA decoys.
  • Combining RNA circles with high-affinity decoy methodologies presents a promising delivery strategy.

Conclusions:

  • Vector-encoded miRNA inhibitors provide refined tools for both research and potential therapeutics.
  • RNA circles represent a novel and potentially effective platform for delivering miRNA inhibitors.
  • Future research may focus on "vectorized" RNA circles for therapeutic miRNA inhibition.