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Factors Influencing Microbial Growth: Temperature01:27

Factors Influencing Microbial Growth: Temperature

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Updated: May 10, 2026

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
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Factors affecting microbial fuel cell acclimation and operation in temperate climates.

I S Michie1, J R Kim, R M Dinsdale

  • 1Faculty of Health, Sport and Science, Sustainable Environment Research Centre SERC, University of Glamorgan, Pontypridd, Mid-Glamorgan, CF37 1DL, UK. imichie@glam.ac.uk

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|June 12, 2013
PubMed
Summary

Microbial fuel cell (MFC) performance depends on anodic biofilm enrichment strategies. Substrate type and temperature significantly impact MFC power density and stability, with acetate-enriched biofilms showing the highest power output.

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Area of Science:

  • Bioelectrochemistry
  • Microbial Ecology
  • Renewable Energy Systems

Background:

  • Scaling up microbial fuel cells (MFCs) requires robust anodic biofilms.
  • Enrichment strategies must optimize performance and environmental resilience.

Purpose of the Study:

  • To investigate how substrate type and temperature affect anodic biofilm enrichment and MFC performance.
  • To assess the stability of MFCs under varying temperature conditions.

Main Methods:

  • Cluster analysis of Denaturing Gradient Gel Electrophoresis (DGGE) community fingerprints.
  • Enrichment of electrogenic biofilms using different substrates (acetate, butyrate, sucrose) and temperatures (20°C, 35°C).
  • Monitoring power density and stability during temperature perturbations.

Main Results:

  • Acetate enrichment yielded the highest power density (7.2 W m⁻³).
  • Sucrose-enriched biofilms exhibited altered substrate responses and lacked recovery after temperature shifts.
  • Biofilms acclimated at 35°C were less stable under temperature perturbations than those acclimated at 20°C.

Conclusions:

  • Anodic biofilm enrichment strategies significantly influence MFC power density and stability.
  • Temperature and substrate type are critical factors for MFC scale-up and reliable operation.
  • Complex substrate metabolism requires robust syntropic interactions for stable MFC performance.