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Related Experiment Video

Updated: May 10, 2026

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

Published on: February 17, 2015

Embryonic stem cell-derived CD166+ precursors develop into fully functional sinoatrial-like cells.

Angela Scavone¹, Daniela Capilupo, Nausicaa Mazzocchi

¹Department of Biosciences, Università degli Studi di Milano, Milano, Italy.

Circulation Research

|June 12, 2013

Summary

Researchers identified CD166 as a marker to isolate sinoatrial node (SAN) progenitor cells from stem cells. These CD166+ cells form functional, autorhythmic SAN-like tissue without forming teratomas.

Keywords:

HCN channels cardiac progenitor cells embryonic stem cells pacemaker sinoatrial node

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Last Updated: May 10, 2026

Generation of Murine Cardiac Pacemaker Cell Aggregates Based on ES-Cell-Programming in Combination with Myh6-Promoter-Selection

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Published on: February 17, 2015

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Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Published on: January 12, 2015

Area of Science:

Cardiovascular Biology
Stem Cell Biology
Regenerative Medicine

Background:

Biological pacemakers require stem cell differentiation and selection of sinoatrial node (SAN) cardiomyocyte properties.
Identifying specific markers for SAN progenitor selection has been a significant challenge.
CD166 is transiently expressed in the developing mouse SAN.

Purpose of the Study:

To investigate the utility of CD166 expression for isolating SAN progenitors from differentiating embryonic stem cells.
To characterize the properties of CD166-selected cardiac precursor cells.

Main Methods:

Embryonic stem cells were differentiated and sorted based on CD166 expression.
Gene expression analysis was performed to identify SAN and ventricular markers.
Autorhythmicity and electrophysiological properties of CD166+ cells were assessed in vitro.
Teratoma formation was evaluated in vivo.

Main Results:

CD166 and HCN4 (a SAN marker) were coexpressed in embryonic day 10.5 mouse hearts.
CD166+ cells expressed high levels of SAN development and function genes, and low levels of ventricular genes.
CD166+ cells formed an autorhythmic syncytium with SAN myocyte-like electrophysiological properties.
CD166-selected cells paced neonatal ventricular myocytes and did not form teratomas in vivo.

Conclusions:

CD166 is a reliable marker for isolating SAN progenitor cells.
A non-teratogenic population of cardiac precursors can be isolated using CD166.
These precursors mature into functional SAN-like tissue.