Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Lysogenic Cycle of Bacteriophages

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...

Transduction

Transduction

Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...

Viral Replication: Lytic Cycle

Viral Replication: Lytic Cycle

Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...

Viral Replication: Lysogenic Cycle

Viral Replication: Lysogenic Cycle

The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects its...

Evolution of Microbial Genome

Evolution of Microbial Genome

Microbial genome evolution is a highly dynamic process shaped by continual gene gain and loss across species and strains. This genomic flexibility allows microorganisms to adapt rapidly to environmental pressures and interactions with other organisms. Central to understanding this diversity is the distinction between the core and pan genomes.The core genome comprises the genes shared by all sampled strains of a species, representing essential functions needed for fundamental cellular processes.

Bacterial Gastroenteritis

Bacterial Gastroenteritis

Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Identification of human DP8α regulatory T cell sub-populations reactive to health-associated anti-inflammatory gut commensals.

Gut microbes·2026

Same author

Pulsed light causes structural damage and proteomic alterations in Aspergillus brasiliensis conidia.

Food microbiology·2026

Same author

[Second day of the interdisciplinary group dedicated to phage therapy and biocontrol].

Virologie (Montrouge, France)·2026

Same author

Role of pattern recognition receptors and microbiota-derived ligands in obesity.

Frontiers in microbiomes·2026

Same author

Glutaminolysis Mediated by the Gut-Muscle Axis Facilitates Collagen Cross-Linking to Regulate Muscle Texture Traits in Tilapia.

Journal of agricultural and food chemistry·2026

Same author

Ornithine lipids from <i>Akkermansia muciniphila</i> are dynamically modulated in colitis and shape macrophage inflammatory responses.

Gut microbes·2025

Same journal

Genetic survey of biomarkers at early and mid-pregnancy identifies pregnancy-specialized immune regulation.

PLoS genetics·2026

Same journal

Argonaute proteins orchestrate Meiotic Sex Chromosome Inactivation and timing of the spermatogenic transcriptional program.

PLoS genetics·2026

Same journal

Genome wide association study meta-analysis of neuropathologic lesions of Alzheimer's disease and related dementias in a multi-site autopsy cohort.

PLoS genetics·2026

Same journal

Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.

PLoS genetics·2026

Same journal

Multiple instance fine-mapping: Predicting causal regulatory variants with a deep sequence model.

PLoS genetics·2026

Same journal

Nuclear ubiquitin-conjugating enzyme TrUbc4 and F-box protein TrFwd1-mediated modification of Cre1 in Trichoderma reesei establishes a regulatory mechanism for carbon catabolite repression.

PLoS genetics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 9, 2026

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Published on: September 1, 2018

Enterococcus faecalis prophage dynamics and contributions to pathogenic traits.

Renata C Matos¹, Nicolas Lapaque, Lionel Rigottier-Gois

¹INRA, UMR1319 Micalis, Jouy-en-Josas, France.

|June 12, 2013

Summary

This summary is machine-generated.

Enterococcus faecalis V583 prophages can excise and produce active phage progeny when exposed to fluoroquinolones. These prophages contribute to bacterial pathogenicity and gene dissemination, particularly in hospital environments.

More Related Videos

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Published on: January 5, 2024

Related Experiment Videos

Last Updated: May 9, 2026

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Published on: September 1, 2018

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics

Published on: January 5, 2024

Area of Science:

Microbiology
Bacteriology
Virology

Background:

Polylysogeny is an evolutionary process where prophages provide fitness and virulence factors.
Enterococcus faecalis V583 is a high-risk strain adapted to hospital environments, carrying seven prophage-like elements.
Understanding prophage activity is crucial for bacterial evolution and infectious disease dynamics.

Purpose of the Study:

To investigate the activity and biological contribution of prophages within the Enterococcus faecalis V583 genome.
To analyze the excision, replication, and packaging capabilities of individual prophages.
To elucidate the interactions between different prophages and their impact on bacterial traits.

Main Methods:

Systematic analysis of prophage excision, replication, and DNA packaging.
Creation of isogenic E. faecalis strains lacking specific prophages.
Investigation of prophage interactions and their effect on virion formation and excision inhibition.
Assessment of prophage-encoded protein roles in bacterial adhesion.

Main Results:

E. faecalis V583 prophages excise and produce active progeny in the presence of fluoroquinolones.
Prophage pp7 (EfCIV583) hijacks capsids from helper phage 1, a novel molecular piracy in Gram-positive bacteria.
Prophages pp1, pp3, and pp5 inhibit the excision of pp4 and pp6.
Prophages encoding platelet-binding-like proteins are involved in adhesion to human platelets.

Conclusions:

E. faecalis V583 prophages play a significant role in bacterial pathogenicity and virulence.
Fluoroquinolone-induced prophage release may explain antibiotic usage correlation with E. faecalis nosocomial success.
Prophages are key mediators of gene dissemination and adaptation in E. faecalis.