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Related Concept Videos

Protein Transport to the Outer Chloroplast Membrane01:11

Protein Transport to the Outer Chloroplast Membrane

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Plant Cell Wall01:07

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Plant cells have a cell wall, a rigid outer covering that protects the cell and provides shape and support. During cell division, a mixture of enzymes, proteins, and glucose molecules is transported via vesicles to the center of the cell. These vesicles continuously fuse and build a cell plate between the dividing cells. As the cell plate matures, new polysaccharides are added to it to form the cell walls of the daughter cells. The predominant polysaccharide in the cell wall is cellulose, made...
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Protein Transport to the Inner Chloroplast Membrane01:18

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Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
Translocation of Proteins into the Mitochondria01:19

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Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
12:07

Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry

Published on: March 24, 2012

Progress toward the tomato fruit cell wall proteome.

Eliel Ruiz-May1, Jocelyn K C Rose

  • 1Department of Plant Biology, Cornell University Ithaca, NY, USA.

Frontiers in Plant Science
|June 12, 2013
PubMed
Summary
This summary is machine-generated.

Investigating the tomato fruit cell wall proteome reveals dynamic protein activity crucial for growth and defense. Diverse methods identify key proteins, including those secreted through non-canonical pathways.

Keywords:
cell wallfruitproteomicssecretiontomato

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Area of Science:

  • Plant Biology
  • Proteomics
  • Biochemistry

Background:

  • The plant cell wall (CW) is a dynamic compartment with a complex proteome essential for cellular structure, growth, and environmental interaction.
  • Tomato fruit development involves significant CW remodeling, making it an ideal model for studying CW-localized proteins.
  • Secreted proteins play vital roles in plant defense against pathogens and abiotic stresses.

Purpose of the Study:

  • To review and discuss orthogonal techniques used to identify proteins within the tomato fruit cell wall and cuticle.
  • To provide insights into the composition and dynamics of the CW proteome during fruit development.
  • To explore emerging issues such as non-canonical protein secretion in plants.

Main Methods:

  • Proteomic analysis targeting the CW and cuticle.
  • Functional secretome screens.
  • Lectin affinity chromatography.
  • Computational prediction of proteins entering the secretory pathway.

Main Results:

  • Multiple techniques collectively enhance the identification of CW-localized proteins.
  • Insights into the dynamic nature of the CW proteome during tomato fruit ontogeny.
  • Evidence supporting the occurrence of non-canonical protein secretion.

Conclusions:

  • Orthogonal approaches provide a comprehensive understanding of the tomato fruit CW proteome.
  • The study highlights the importance of secreted proteins in fruit development and defense.
  • Further research is needed to fully elucidate the mechanisms and prevalence of non-canonical protein secretion.