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Tin oxide quantum dot based DNA sensor for pathogen detection.

Manoj K Patel¹, Jay Singh, Manish K Singh

¹National Physical Laboratory (CSIR), Department of Science and Technology, Centre on Biomolecular Electronics, Biomedical Instrumentation Section, Dr. K. S. Krishnan Marg, New Delhi 110012, India.

Journal of Nanoscience and Nanotechnology

|June 13, 2013

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Nano crystalline tin oxide quantum dots (SnO2-QDs) enable sensitive electrochemical detection of Vibrio cholerae using DNA hybridization. This novel bioelectrode offers a rapid, stable, and low-limit detection method for bacterial pathogens.

Area of Science:

Nanotechnology
Biosensors
Electrochemistry

Background:

Vibrio cholerae poses a significant public health threat, necessitating rapid and accurate detection methods.
Current diagnostic techniques can be time-consuming and require specialized laboratory equipment.
Development of sensitive and specific biosensors is crucial for early identification and control of cholera outbreaks.

Purpose of the Study:

To develop a novel electrochemical biosensor for the detection of Vibrio cholerae.
To utilize nano crystalline tin oxide quantum dots (SnO2-QDs) for enhanced DNA hybridization detection.
To evaluate the sensitivity, specificity, and stability of the fabricated bioelectrode.

Main Methods:

Synthesis of SnO2-QDs (1-5 nm) via laser ablation in liquid (LAL).

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Electrophoretic deposition of SnO2-QDs onto indium tin oxide (ITO) coated glass electrodes.

Immobilization of a specific oligonucleotide probe for Vibrio cholerae virulent gene onto the SnO2-QDs/ITO surface.

Electrochemical detection of DNA hybridization using varying genomic DNA concentrations.

Main Results:

The SnO2-QDs/ITO bioelectrode demonstrated effective binding with DNA phosphate groups, enhancing electron transport.
The hybridized electrode exhibited a linear response (R=0.974) across a range of DNA concentrations (100-500 ng/µL).
High sensitivity (35.20 nA/ng/cm²), a low detection limit (31.5 ng/µL), and a fast response time (3 s) were achieved.
The bioelectrode maintained high stability for 0-120 days under refrigerated storage.

Conclusions:

Nano crystalline tin oxide quantum dots are effective for electrochemical detection of Vibrio cholerae via DNA hybridization.
The developed bioelectrode offers a sensitive, rapid, and stable platform for bacterial detection.
This approach holds promise for improved diagnostics of infectious diseases.