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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

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Published on: March 13, 2018

Solution-based circuits enable rapid and multiplexed pathogen detection.

Brian Lam1, Jagotamoy Das, Richard D Holmes

  • 1Department of Chemistry, Faculty of Arts and Sciences, University of Toronto, Toronto, Ontario, Canada M5S 3M2.

Nature Communications
|June 13, 2013
PubMed
Summary

This study introduces a novel solution-based circuit for highly multiplexed electrochemical sensing on passive chips. This advance enables rapid and accurate analysis of unpurified samples for disease biomarkers and pathogen identification.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Electronic readout offers sensitive biomarker detection but struggles with large panels.
  • Interrogating numerous biomarkers is crucial for advanced diagnostics like pathogen profiling.
  • Addressing large sensor arrays on affordable platforms remains a significant challenge.

Purpose of the Study:

  • To develop a novel approach for highly multiplexed electrochemical sensing on passive chips.
  • To overcome limitations in addressing large electrode-based sensor arrays.
  • To enable sensitive and specific detection of numerous disease biomarkers simultaneously.

Main Methods:

  • Development of a "solution-based circuits formed on chip" concept.
  • Implementation of signal readout channel switching to eliminate crosstalk.
  • Fabrication and testing of chips with biomolecule-specific microsensors.

Main Results:

  • Demonstrated feasibility of highly multiplexed electrochemical sensing on passive chips.
  • Successfully analyzed unpurified clinical samples with high accuracy.
  • Accurately classified pathogens at clinically relevant concentrations.
  • Achieved accurate signature molecule readout within 2 minutes of sample introduction.

Conclusions:

  • The developed solution-based circuits enable multiplexed electrochemical sensing on inexpensive platforms.
  • This technology facilitates accurate analysis of unpurified samples for disease diagnostics.
  • Rapid and sensitive detection of multiple biomarkers is now feasible for advanced disease profiling.