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Required characteristics of Paenibacillus polymyxa JB-0501 as potential probiotic

Karim Naghmouchi1, John Baah, Benoit Cudennec

  • 1Lethbridge Research Center, Agriculture and Agri-Food Canada, AB, Canada. karim.naghmouchi@gmail.com

Archives of Microbiology
|June 13, 2013
PubMed

Insights

Paenibacillus polymyxa JB-0501 shows probiotic potential by inhibiting E. coli growth and adhering to Caco-2 cells. This strain

Area of Science:

  • Microbiology
  • Animal Science
  • Probiotics

Background:

  • Paenibacillus polymyxa is a bacterium found in livestock feed supplements.
  • Evaluating probiotic properties is crucial for animal feed additives.

Purpose of the Study:

  • To assess the probiotic potential of Paenibacillus polymyxa JB-0501.
  • To compare JB-0501 with reference strains regarding stress resistance and adherence.
  • To determine the inhibitory effect on Escherichia coli growth.

Main Methods:

  • Tested carbohydrate utilization and resistance to lysozyme, acid, bile salts, and hydrogen peroxide.
  • Assessed adherence to Caco-2 cell monolayers.
  • Evaluated inhibition of Escherichia coli ATCC 25922 growth using agar diffusion tests.

Main Results:

  • P. polymyxa JB-0501 demonstrated growth at pH 4.5 and low H2O2 concentrations.
  • Both P. polymyxa JB-0501 and P. polymyxa ATCC 43865 adhered to Caco-2 cells.
  • P. polymyxa JB-0501 effectively inhibited E. coli ATCC 25922 growth.

Conclusions:

  • P. polymyxa JB-0501 exhibits significant probiotic properties.
  • Its ability to inhibit E. coli and adhere to intestinal cells supports its use in livestock feed.

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