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[Preparation of cationic vancomycin hydrochloride multivesicular liposomes and its quality]
Duming Yang1, Yongqing Xu, Fubing Li
1Department of Orthopaedics, Kunming General Hospital of Chengdu Minitary Command, the Orthopaedic Trauma Institute of Chinese PLA, Kunming Yunnan, 650032, P.R.China.
Objective:
To prepare cationic Vancomycin hydrochloride multivesicular liposome (MVL) and to inspect its quality.
Methods:
Cationic Vancomycin hydrochloride MVLs were prepared by double emulsion method, and the storing solution of Vancomycin was prepared. The analysis method of Vancomycin in vitro was established; the specificity, precision, and resorption rate were estimated. Reverse phase high performance liquid chromatography (RP-HPLC) was used to determine the concentration of Vancomycin, encapsulation efficiency, and release characteristics in vitro. The formulation and pharmaceutical process were optimized by single factor experiments and orthogonal experimental design with the factor of encapsulation efficiency as the criteria. The liposome morphology was observed by optical microscopy and transmission electron microscopy. The particle size and Zeta potential were determined by Malvern instrument. The stability was analyzed by dynamic analysis.
Results:
An RP-HPLC method was established for the assay of Vancomycin. The analysis method was precise, simple, and reliable for the quality control of Vancomycin. Vancomycin hydrochloride MVLs were round and well-distributed. The average particle size and the encapsulation efficiency were 3.3 microm and 24.9%, respectively. Zeta potential was 24.53 mV, and 90.5% of Vancomycin hydrochloride was released after 264 hours in normal saline under 37 degrees C. Cationic Vancomycin MVLs were stored for 1 month at 4 degrees C, which mantained good stability.
Conclusion:
Cationic Vancomycin hydrochloride MVLs have good appearance, high encapsulation efficiency, good stability, and significant sustained release properties.
