Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

miR-146a Mimic Therapy Protects Against Platelet-Mediated Thrombosis.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Efficacy and safety of recombinant activated factor VII for secondary prophylaxis in patients with Glanzmann thrombasthenia.

Research and practice in thrombosis and haemostasis·2026
Same author

Corrigendum to 'A Novel Homozygous Splice-Site Variant in VPS33B Identified as a Cause of Bleeding': [JTHA 24/1, January 2026, Pages 146-154].

Journal of thrombosis and haemostasis : JTH·2026
Same author

Platelet biology and etiopathogenesis of congenital platelet disorders.

Blood reviews·2026
Same author

Cocaine Upregulates Microglial Lipid Droplet Formation Through Increasing Lipid Synthesis Activity In Vitro and In Vivo.

Biomolecules·2026
Same author

Fear of recurrence, secondary cancers, and health problems in long-term survivors of childhood cancer: Findings from a Canadian cohort.

Cancer·2026

Related Experiment Video

Updated: May 10, 2026

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
09:19

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Bioactive hydrogels with enhanced initial and sustained cell interactions.

Mary Beth Browning1, Brooke Russell, Jose Rivera

  • 1Department of Biomedical Engineering, Texas A&M University, 5045 Emerging Technologies Building, College Station, Texas 77843-3120, United States.

Biomacromolecules
|June 14, 2013
PubMed
Summary

Reducing poly(ethylene glycol) (PEG) linker density on proteins improves cell adhesion in bioactive hydrogels. A novel, stable PEG linker (Aam-PEG-I) enhances cell interactions and maintains bioactivity over time.

More Related Videos

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
08:50

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

Related Experiment Videos

Last Updated: May 10, 2026

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
09:19

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Published on: September 15, 2017

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications
08:50

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Published on: August 4, 2017

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications
09:30

The Synthesis of RGD-functionalized Hydrogels as a Tool for Therapeutic Applications

Published on: October 7, 2016

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Drug Delivery

Background:

  • Poly(ethylene glycol) (PEG)-based hydrogels offer tunable properties for regenerative medicine and drug delivery.
  • PEG hydrogels resist protein adsorption and cell adhesion, enabling controlled bioactive factor incorporation.
  • Acrylate-PEG-N-hydroxysuccinimide (Acr-PEG-NHS) is a common PEG linker, but can hinder cell-material interactions.

Purpose of the Study:

  • To investigate the impact of PEG linker density on cell adhesion and protein retention in bioactive hydrogels.
  • To develop a novel PEG linker with improved hydrolytic stability for enhanced cell-material interactions.

Main Methods:

  • Functionalization of bioactive factors with varying densities of Acr-PEG-NHS and a novel acrylamide-PEG-isocyanate (Aam-PEG-I) linker.
  • Assessment of cell adhesion, spreading, and protein retention within PEG hydrogel networks.
  • Evaluation of the hydrolytic stability of the novel Aam-PEG-I linker.

Main Results:

  • Reducing Acr-PEG-NHS linker density significantly improved cell adhesion and spreading.
  • Lowering Acr-PEG-NHS density led to increased protein loss from the hydrogel matrix due to ester hydrolysis.
  • The novel Aam-PEG-I linker demonstrated enhanced hydrolytic stability.
  • Decreased Aam-PEG-I linker density improved cell adhesion comparable to Acr-PEG-NHS systems while maintaining protein levels.

Conclusions:

  • Optimizing PEG linker density is crucial for balancing cell interactions and bioactivity in hydrogels.
  • The novel Aam-PEG-I linker offers improved hydrolytic stability, enabling sustained cell-material interactions and bioactivity.
  • Aam-PEG-I provides a new strategy for protein functionalization in applications requiring enhanced and prolonged cell-material engagement.