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Related Experiment Video

Updated: May 10, 2026

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
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Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Published on: December 23, 2022

Predicting therapy response in live tumor cells isolated with the flexible micro spring array device.

Jean-Nicolas Gallant1, Elizabeth M Matthew, Hairong Cheng

  • 1Laboratory of Translational Oncology and Experimental Cancer Therapeutics, Division of Hematology-Oncology, Penn State Hershey Cancer Institute; Hershey, PA, USA.

Cell Cycle (Georgetown, Tex.)
|June 14, 2013
PubMed
Summary

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A novel microfluidic device, the flexible micro spring array (FMSA), effectively isolates and expands rare circulating tumor cells (CTCs) from blood samples. This technology enables reliable chemosensitivity testing and the study of CTC biology for cancer treatment development.

Area of Science:

  • Oncology
  • Biotechnology
  • Microfluidics

Background:

  • Circulating tumor cells (CTCs) shed from primary tumors into the bloodstream.
  • Monitoring CTCs aids in assessing metastasis and treatment response.
  • Current methods for CTC isolation and expansion face challenges with rare and viable cells.

Purpose of the Study:

  • To demonstrate the utility of the flexible micro spring array (FMSA) device for CTC enrichment.
  • To establish a method for expanding and characterizing viable CTCs.
  • To evaluate the potential of FMSA-derived CTCs for chemosensitivity testing and biological studies.

Main Methods:

  • Utilized the FMSA device, a microfluidic tool enriching CTCs based on size and deformability.
  • Spiked cancer cells into healthy donor blood to simulate patient samples.
Keywords:
circulating tumor cellsdrug sensitivity testingmicrofluidicpersonalized medicineviable cell capture

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  • Recovered and expanded isolated cells in vitro and in vivo.
  • Performed chemosensitivity assays on expanded CTC cultures.

    • Main Results:

    • The FMSA device successfully isolated CTCs from spiked blood samples.
    • Isolated cells were expanded in vitro and in vivo, demonstrating viability.
    • As few as 20 colon cancer cells in 7.5 mL blood were isolatable and expandable.
    • Reliable chemosensitivity data was obtained using as few as 25 cells per well.
    • Viable patient CTCs were isolated and maintained in culture for several weeks.

    Conclusions:

    • The FMSA device offers a novel and effective method for isolating viable CTCs from human blood.
    • This technology facilitates the study of rare CTC biology and drug efficacy testing.
    • The FMSA device holds promise for personalized cancer treatment strategies and clinical trials.