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Updated: May 10, 2026

Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations
Published on: May 31, 2020
Cytotoxic and anti-inflammatory metabolites from the soft coral Scleronephthya gracillimum
Hui-Yu Fang1, Chi-Hsin Hsu, Chih-Hua Chao
1Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
Abstract:
Five new pregnane-type steroids, sclerosteroids J-N (1-5), and a diterpenoid with a new chemotype 3-methyl-5-(10'-acetoxy-2',6',10'-trimethylundecyl)-2-penten-5-olide (6), have been isolated from a soft coral Scleronephthya gracillimum. The structures of the metabolites were determined by extensive spectroscopic analysis. Compound 4 exhibited cytotoxicity against HepG2, A549, and MDA-MB-231 cancer cell lines. Furthermore, steroids 2 and 4 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS protein, and 1, 2, 4 and 5 could effectively reduce the accumulation of COX-2 protein in LPS-stimulated RAW264.7 macrophage cells.
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