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Related Concept Videos

Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

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Related Experiment Video

Updated: May 10, 2026

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells
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Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Published on: July 18, 2017

Cartilage tissue engineering identifies abnormal human induced pluripotent stem cells.

Akihiro Yamashita1, Shiying Liu, Knut Woltjen

  • 1Department of Biochemistry & Molecular Biology, University of Calgary, Calgary, Alberta, Canada.

Scientific Reports
|June 14, 2013
PubMed
Summary

A new method using in vitro cartilage tissue engineering can identify tumorigenic potential in human induced pluripotent stem cells (iPSCs). This approach screens human iPSC lines for safety, detecting abnormal clones that could pose oncogenic risks in cell therapies.

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Last Updated: May 10, 2026

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Oncology

Background:

  • Ensuring the safety of human cell therapies is paramount.
  • Human induced pluripotent stem cells (iPSCs) lack a reliable safety indicator for tumorigenic potential.
  • Previous studies in chimeric mice showed tumorigenicity in some iPSC lines, but a human iPSC screen is needed.

Purpose of the Study:

  • To establish an in vitro method for screening human iPSC lines for tumorigenic potential.
  • To identify abnormal human iPSC lines that may pose safety risks in cell-based therapies.

Main Methods:

  • Utilized in vitro cartilage tissue engineering as a screening tool for human iPSC lines.
  • Differentiated human embryonic stem cells (ESCs) and various human iPSC lines in cartilage constructs.
  • Monitored for the development of secretory tumors during in vitro differentiation as an indicator of tumorigenicity.

Main Results:

  • All tested human ESCs and most human iPSC lines safely formed cartilage.
  • Certain human iPSC lines exhibited a pro-oncogenic state, forming secretory tumors in vitro.
  • Five abnormal iPSC clones were identified among 21 lines derived from diverse reprogramming methods and cellular origins.

Conclusions:

  • In vitro cartilage tissue engineering serves as an effective method for identifying abnormal human iPSC lines.
  • This approach can detect tumorigenic potential in human iPSCs, enhancing cell therapy safety.
  • The study highlights the importance of rigorous safety screening for iPSC-based clinical applications.