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The PIM-2 kinase is an essential component of the ultraviolet damage response that acts upstream to E2F-1 and ATM

Shahar Zirkin1, Ateret Davidovich, Jeremy Don

  • 1Mina & Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 5290002, Israel.

The Journal of Biological Chemistry
|June 14, 2013
PubMed

Insights

The PIM-2 kinase enhances cell survival after UV damage by aiding DNA repair. PIM-2 (PIM-2 kinase) acts upstream of E2F-1 and ATM in the UV DNA damage response pathway.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • The PIM-2 kinase is known for its pro-survival and anti-apoptotic roles through substrate phosphorylation.
  • Pro-survival effects can also be mediated by enhanced DNA repair mechanisms following cellular damage.
  • The role of PIM-2 in response to UV-induced DNA damage has not been previously investigated.

Purpose of the Study:

  • To investigate the potential role of PIM-2 in the cellular response to UV radiation.
  • To determine if PIM-2 influences DNA repair efficiency and cell viability after UV exposure.

Main Methods:

  • U2OS cells were exposed to UVC radiation.
  • PIM-2 expression and activity were monitored.
  • PIM-2 was silenced or overexpressed.
  • DNA lesion removal, γH2AX accumulation, and cell viability were assessed.
  • The roles of E2F-1 and ATM were evaluated through silencing and inhibition.

Main Results:

  • PIM-2 expression and activity increased following UVC exposure.
  • PIM-2-silenced cells exhibited increased sensitivity to UV radiation.
  • PIM-2 overexpression accelerated UV-induced DNA lesion removal and reduced γH2AX accumulation.
  • Overexpression of PIM-2 enhanced cell viability post-UV radiation.
  • The protective effects of PIM-2 were dependent on E2F-1 and ATM activation.

Conclusions:

  • PIM-2 plays a significant role in the cellular response to UV-induced DNA damage.
  • PIM-2 promotes cell survival following UV exposure by enhancing DNA repair.
  • PIM-2 functions upstream of E2F-1 and ATM in the UV DNA damage response pathway.
Keywords:
ATMApoptosisBase Excision RepairCancer BiologyCell SignalingDNA Damage ResponseE2F-1PIM-2

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