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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: May 10, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Frequency multiplexed long range swept source optical coherence tomography.

Mantas Zurauskas1, Adrian Bradu, Adrian Gh Podoleanu

  • 1Applied Optics Group, School of Physical Sciences, University of Kent, CT2 7NH Canterbury, UK.

Biomedical Optics Express
|June 14, 2013
PubMed
Summary
This summary is machine-generated.

A new swept source optical coherence tomography system uses acousto-optic deflectors to capture multiple B-scans simultaneously. This innovation enables enhanced depth imaging and long-range B-scan visualization in biological samples.

Keywords:
(110.4500) Optical coherence tomography(110.6880) Three-dimensional image acquisition(120.3180) Interferometry(140.3600) Lasers, tunable(170.0110) Imaging systems(170.1065) Acousto-optics

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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

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Last Updated: May 10, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
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Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Area of Science:

  • Biomedical optics
  • Optical imaging technologies
  • Advanced microscopy techniques

Background:

  • Optical Coherence Tomography (OCT) is a valuable non-invasive imaging modality.
  • Swept-source OCT (SS-OCT) offers advantages in speed and imaging depth.
  • Simultaneous acquisition of multiple B-scans can improve imaging efficiency and depth coverage.

Purpose of the Study:

  • To introduce a novel SS-OCT configuration utilizing acousto-optic deflectors (AODs).
  • To evaluate the sensitivity range of the multi-B-scan acquisition system.
  • To demonstrate the capability for long-range B-scan imaging using the developed configuration.

Main Methods:

  • Implementation of a swept-source OCT system incorporating AODs.
  • Simultaneous acquisition of five B-scans at different depths.
  • Evaluation of system sensitivity across the acquired B-scans.
  • Application of the system for long-range imaging of a mouse head sample by combining two B-scans.

Main Results:

  • The novel SS-OCT configuration successfully acquired multiple B-scans simultaneously.
  • Sensitivity range was assessed during the acquisition of five simultaneous B-scans.
  • Long-range B-scan imaging was demonstrated on a mouse head sample.
  • The system proved effective in combining simultaneous B-scans for extended visualization.

Conclusions:

  • The developed SS-OCT configuration with AODs enables simultaneous multi-B-scan acquisition.
  • This approach enhances imaging capabilities for deeper structures and longer ranges.
  • The technology shows promise for advanced biomedical imaging applications.