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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
Published on: March 24, 2016
Mirion--a software package for automatic processing of mass spectrometric images.
C Paschke1, A Leisner, A Hester
1Institute for Inorganic and Analytical Chemistry, Justus Liebig University Giessen, 35392, Giessen, Germany.
Journal of the American Society for Mass Spectrometry
|June 14, 2013
Summary
Mass spectrometric imaging (MSI) data processing is challenging. The free Mirion software simplifies the analysis of large MSI datasets by enabling efficient image generation and comparison of compounds.
Area of Science:
- Life Sciences
- Analytical Chemistry
- Bioinformatics
Background:
- Mass spectrometric imaging (MSI) generates large datasets requiring efficient analysis.
- Manual interpretation of MSI data is time-consuming and impractical.
- Visualizing MSI data as spatial distribution images aids interpretation.
Purpose of the Study:
- To present Mirion, a free software package for handling and analyzing MSI data.
- To address the bottleneck in MSI data processing through automated image generation.
- To facilitate the comparison of different analytes within MSI datasets.
Main Methods:
- Development of the Mirion software package.
- Utilizing the imzML standard data format for broad instrument compatibility.
- Implementing automated image generation, calculation, and overlay functionalities.
Main Results:
- Mirion enables efficient processing and analysis of large MSI datasets.
- The software supports data from various mass spectrometer sources via imzML.
- Automated image generation and overlay facilitate direct comparison of analytes.
Conclusions:
- Mirion provides a user-friendly solution for MSI data interpretation.
- The software streamlines the transformation of complex MSI data into visualizable images.
- Mirion enhances the efficiency and accessibility of mass spectrometric imaging analysis.
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