Related Experiment Video
Updated: May 10, 2026
10:45
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
Published on: May 25, 2017
Liver transcriptome changes in zebrafish during acclimation to transport-associated stress
Anusha K S Dhanasiri1, Jorge M O Fernandes, Viswanath Kiron
1Faculty of Biosciences and Aquaculture, University of Nordland, Bodø, Norway.
Plos One
|June 14, 2013
Summary
Transport significantly alters zebrafish liver gene expression, impacting energy metabolism, ammonia regulation, and immune function. These molecular adjustments are crucial for maintaining homeostasis during stress acclimation.
Area of Science:
- * Comparative genomics and molecular biology.
- * Aquatic animal physiology and stress response.
Background:
- * The liver is vital for stress acclimation in fish.
- * Understanding molecular changes in zebrafish liver during transport is key to assessing their health and welfare.
Purpose of the Study:
- * To investigate the liver transcriptome of zebrafish during simulated transport.
- * To identify molecular pathways and genes affected by transport stress.
Main Methods:
- * Oligonucleotide array (44 K) analysis of liver total RNA from zebrafish.
- * Samples collected immediately after packing (0 h), and at 48 and 72 h during mock transport.
Main Results:
- * Widespread gene regulation observed, particularly at 48/72 h, affecting gluconeogenesis, glycolysis, fatty acid metabolism, and ammonia regulation.
- * Upregulation of ubiquitination and MAPK signaling, downregulation of ROS generation and xenobiotic metabolism observed.
- * Modulation of stress and thyroid hormone-related transcripts, alongside suppression of immune genes.
Conclusions:
- * Zebrafish liver undergoes complex molecular adjustments to cope with transport stress.
- * These changes highlight alterations in energy utilization, detoxification, and immune status.
- * The study provides insights into the physiological impact of transport on fish health.
