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Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Body Composition and Metabolic Caging Analysis in High Fat Fed Mice

Published on: May 24, 2018

Methods and models for metabolic assessment in mice.

G Pacini¹, B Omar, B Ahrén

¹Metabolic Unit, ISIB CNR, Padua, Italy. giovanni.pacini@isib.cnr.it

Journal of Diabetes Research

|June 14, 2013

Summary

Mice are ideal models for type 2 diabetes research, offering reproducible metabolic assessments. This review details key tests for evaluating insulin resistance and secretion in mice, crucial for developing new therapies.

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Area of Science:

* Metabolic research and preclinical drug development for type 2 diabetes.
* In vivo experimental systems and animal modeling in endocrinology.

Background:

* Development of novel type 2 diabetes therapies necessitates validated in vivo models.
* Mice offer advantages as a model organism due to their physiology and genetics.

Purpose of the Study:

* To summarize the execution and data analysis of metabolic assessment tests in mice.
* To provide a guide for reliable measurement of insulin resistance and beta-cell function.
* To detail methods for evaluating potential type 2 diabetes treatments in mice.

Main Methods:

* Review of key metabolic assessment tests: glucose clamp, intravenous glucose tolerance test (IVGTT), and oral glucose tolerance test (OGTT).
* Description of experimental performance, advantages, and limitations for each test.

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* Explanation of empirical formulas and mathematical models for data analysis.

Main Results:

* Provides a framework for reproducible assessment of insulin sensitivity and secretion in mice.
* Details analytical approaches to ensure reliable interpretation of metabolic data.
* Highlights the utility of mouse models in diabetes research.

Conclusions:

* Standardized metabolic testing in mice is essential for advancing type 2 diabetes therapies.
* The described methods and analytical techniques enable robust evaluation of drug efficacy.
* This review serves as a comprehensive resource for researchers utilizing mouse models in diabetes studies.