Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering drug-responsive replication machinery for precise control of self-amplifying RNA.

Nature biomedical engineering·2026
Same author

Current Progress and Future Outlook for Synthetic Gene Circuits in Cardiovascular Therapy.

Biomolecules·2026
Same author

ETV2 Mediated Differentiation of Human Pluripotent Stem Cells Results in Functional Endothelial Cells for Engineering Advanced Vascularized Microphysiological Models.

Advanced healthcare materials·2026
Same author

Reversing transgene silencing via targeted chromatin editing.

bioRxiv : the preprint server for biology·2025
Same author

Analog epigenetic memory revealed by targeted chromatin editing.

Cell genomics·2025
Same author

Development of a Lentiviral Vector for High-Yield Production of Synthetic and Recombinant GCase for Gaucher Disease Therapy.

International journal of molecular sciences·2025
Same journal

Breaking the Stability-Activity-Selectivity Trilemma in Unspecific Peroxygenase through Computation-Based Cross-Regional Combinatorial Mutagenesis.

ACS synthetic biology·2026
Same journal

Sequential Plasmid Curing and Genome Editing in <i>Escherichia coli</i> Nissle 1917.

ACS synthetic biology·2026
Same journal

An Explainable Deep Learning Framework Integrating DNA Sequence and Transcription Initiation Signals for Gene Expression Prediction.

ACS synthetic biology·2026
Same journal

A Multitask Prediction Framework for CircRNAs, Drugs, and Diseases Based on Multi-View Information Integration and Graph Contrastive Learning.

ACS synthetic biology·2026
Same journal

Engineering Modular Cargo Loading Strategies for Carboxysome-Derived Protein Particles.

ACS synthetic biology·2026
Same journal

Suppression of Salmonella Effectors with CRISPRi Controls the Immune Response to Bacterial Therapies.

ACS synthetic biology·2026
See all related articles

Related Experiment Video

Updated: May 10, 2026

Genetic Manipulation in &Delta;ku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
09:52

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

Genetically programmable pathogen sense and destroy.

Saurabh Gupta1, Eran E Bram, Ron Weiss

  • 1Department of Biological Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

ACS Synthetic Biology
|June 15, 2013
PubMed
Summary
This summary is machine-generated.

Engineered E. coli detect Pseudomonas aeruginosa (P. aeruginosa) using quorum sensing molecules. These E. coli sentinels secrete a novel bacteriocin, CoPy, to specifically kill P. aeruginosa, offering a potential cell therapy for infections.

More Related Videos

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
09:29

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies
11:12

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

Related Experiment Videos

Last Updated: May 10, 2026

Genetic Manipulation in &Delta;ku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
09:52

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Published on: July 12, 2013

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation
09:29

Electroporation-Based CRISPR-Cas9-Mediated Gene Knockout in THP-1 Cells and Single-Cell Clone Isolation

Published on: February 28, 2025

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies
11:12

Immunometabolic Circuits in Infection for Advancing Host Directed Therapies

Published on: September 13, 2024

Area of Science:

  • Synthetic biology
  • Microbiology
  • Bacteriocin research

Background:

  • Pseudomonas aeruginosa (P. aeruginosa) is a significant pathogen causing urinary tract and nosocomial infections.
  • Current treatments face challenges due to P. aeruginosa's resilience and potential for resistance.

Purpose of the Study:

  • To develop a novel cell therapy approach for targeting P. aeruginosa infections.
  • To engineer a bacterial sentinel system for pathogen detection and elimination.

Main Methods:

  • Genetically modified E. coli to detect P. aeruginosa via quorum sensing molecule 3OC 12 HSL.
  • Engineered E. coli to secrete a chimeric bacteriocin, FlgM-CoPy, specifically targeting P. aeruginosa.
  • Constructed CoPy by combining domains from Colicin E3 and Pyocin S3.

Main Results:

  • Engineered E. coli successfully detected P. aeruginosa and secreted FlgM-CoPy.
  • FlgM-CoPy demonstrated specific toxicity against P. aeruginosa (PAO1), sparing other tested bacteria.
  • Co-culture experiments showed engineered E. coli effectively inhibited P. aeruginosa growth on agar plates.

Conclusions:

  • Proof-of-principle for a synthetic biology-based cell therapy against P. aeruginosa established.
  • The engineered E. coli sentinel system shows promise for targeted eradication of P. aeruginosa infections.
  • Further research into bacteriocin-based therapies for infectious diseases is warranted.