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Published on: March 29, 2018
Adenovirus encoding BMP-7 immobilized on titanium surface exhibits local delivery ability and regulates osteoblast
Shenyuan Chen1, Jiahua Yang, Hang Wang
1State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, #14 Sec. 3 Renmin Road South, Chengdu, PR China.
Objective:
The local delivery of growth factors such as bone morphogenetic protein-7 (BMP-7) into the tissues around dental implants may improve their osseointegration. We have designed a new method of attaching BMP-7 to a titanium surface and assessed both the retention of the BMP-7 and its effect on osteoblast differentiation.
Design:
Adenoviral vector expressing BMP-7 was attached to dental titanium discs by hexon-specific antibodies in a type I collagen-avidin gel. FITC-labelled secondary antibody was used to measure the continuing adherence of the coating after repeated rinsing. Osteoblasts were harvested and seeded on the titanium discs. Gene transduction efficiency and targeting ability were assessed after 24h. Surface morphology was observed by SEM. Cell proliferation and alkaline phosphatase (ALP) activities were measured.
Results:
The anti-adenohexon antibody adhered strongly to the collagen-avidin gels. BMP-7 gene expression was localized precisely to cells growing on the gels bound by the hexon-specific antibody. Osteoblasts on the titanium containing Ad-BMP-7 had a higher ALP activity than those without Ad-BMP-7.
Conclusions:
This study describes a novel technique for the precise attachment of BMP-7 to titanium surfaces. The process may enhance the osseointegration of dental implants.
