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Updated: May 10, 2026

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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016
Next generation sequencing applications in cancer research
Wei Zhang1, Matti Nykter, Kexin Chen
1Department of Pathology, University of Texas, MD Anderson Cancer Center, Houston, Texas 77030, USA; Institute of Biomedical Technology, University of Tampere, Tampere 33014, Finland; Department of Epidemiology and Biostatistics, Tianjin Medical University, Cancer Institute and Hospital, Tianjin 300060, P.R. China.
Cancer Letters
|June 15, 2013
Summary
No abstract available in PubMed .
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Next-generation Sequencing
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

