Related Experiment Video
Updated: May 10, 2026

Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Published on: September 11, 2017
Short-time movement of E. coli chromosomal loci depends on coordinate and subcellular localization
Avelino Javer1, Zhicheng Long, Eileen Nugent
1Cavendish Laboratory, University of Cambridge, Cambridge CB3 0HE, UK.
Abstract:
In bacteria, chromosomal architecture shows strong spatial and temporal organization, and regulates key cellular functions, such as transcription. Tracking the motion of chromosomal loci at short timescales provides information related to both the physical state of the nucleo-protein complex and its local environment, independent of large-scale motions related to genome segregation. Here we investigate the short-time (0.1-10 s) dynamics of fluorescently labelled chromosomal loci in Escherichia coli at different growth rates. At these timescales, we observe for the first time a dependence of the loci's apparent diffusion on both their subcellular localization and chromosomal coordinate, and we provide evidence that the properties of the chromosome are similar in the tested growth conditions. Our results indicate that either non-equilibrium fluctuations due to enzyme activity or the organization of the genome as a polymer-protein complex vary as a function of the distance from the origin of replication.
More Related Videos
Related Concept Videos
Chemotaxis in E. coli
Coordination of Gene Expression Processes in Bacteria
Stringent Response in E. coli
Intracellular Movement of Viruses and Bacteria
Chromatin Position Affects Gene Expression
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Bacterial Gastroenteritis

