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A novel method for the analysis of 3-mercaptopyruvate using high-performance liquid chromatography with fluorescence

Yuki Ogasawara1, Tomoaki Hirokawa, Kaori Matsushima

  • 1Department of Hygienic Chemistry, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan. yo@my-pharm.ac.jp

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|June 15, 2013
PubMed

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...