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DNA-templated nanoantennas for single-molecule detection at elevated concentrations.

Guillermo P Acuna¹, Phil Holzmeister, Friederike M Möller

¹Technische Universität Braunschweig, Physical and Theoretical Chemistry—NanoBioScience, Hans-Sommer-Strasse 10, 38106 Braunschweig, Germany. g.acuna@tu-bs.de

Journal of Biomedical Optics

|June 15, 2013

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Bottom-up nanoantennas boost fluorescence intensity for single-molecule measurements. This breakthrough enables high-concentration analysis, expanding applications in areas like DNA sequencing.

Area of Science:

Nanotechnology
Biophysics
Optical Engineering

Background:

Single-molecule fluorescence techniques face limitations in dynamic concentration range.
Enhancing fluorescence intensity is crucial for expanding the applicability of these methods.

Purpose of the Study:

To develop and demonstrate bottom-up nanoantennas for enhancing fluorescence intensity.
To enable single-molecule measurements at significantly higher concentrations.

Main Methods:

Utilizing self-assembled DNA origami structures as precise platforms for positioning gold nanoparticle (NP) dimers.
Fabricating nanoantennas with controlled nanometer precision using gold NPs with specific dimensions and interparticle gaps.

Main Results:

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Achieved a maximum of nearly 100-fold fluorescence intensity enhancement.
Demonstrated successful single-molecule measurements at concentrations up to 500 nM, a two-order-of-magnitude increase.
Experimental results showed good agreement with numerical simulations.

Conclusions:

Bottom-up nanoantennas, integrated with DNA origami, significantly enhance fluorescence intensity.
This approach overcomes concentration limitations in single-molecule fluorescence, facilitating high-concentration assays like DNA sequencing.
The developed enhancement chambers offer a cost-effective solution for advanced biological applications.