Real-time monitoring of DNA hybridization kinetics on living cell surfaces
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Updated: May 10, 2026

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
Published on: March 23, 2010
Ambadas B Rode1, Tamaki Endoh, Hisae Tateishi-Karimata
1Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, 7-1-20 Minatojima-minamimachi, Kobe 650-0047, Japan.
Abstract:
We determined hybridization rates of DNA probes bound to the surfaces of a human cell, and compared the rates with those determined in solution. The rates were slower on the cell surface than in solution-phase. The position of the nucleation site but not the location of the formed duplex relative to the cell surface adversely affected the hybridization kinetics.
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