Related Experiment Video
Updated: May 10, 2026
11:38
GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
Published on: October 24, 2011
Enzymatic characterization of recombinant enzymes of O-GlcNAc cycling
1Laboratory Cell Biochemistry and Biology, NIDDK, National Institutes of Health, Bethesda, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 15, 2013
Abstract:
The dynamic addition of O-GlcNAc to target proteins is now recognized as a major signaling paradigm impacting phosphorylation, protein turnover, gene expression, and other posttranslational modifications influencing epigenetics. Here we describe the production of and methods for assay of the recombinant enzymes of O-GlcNAc cycling: O-linked GlcNAc Transferase (OGT) and O-GlcNAcase (OGA).
