New violet-excitable reagents for multicolor flow applications

Area of Science:

• Immunology
• Biotechnology
• Analytical Chemistry

Background:

• Violet-excitable dyes are crucial for multicolor flow cytometry (FCM).
• Existing violet dyes have limitations in spectral coverage and compatibility.
• BD Biosciences offers AmCyan and has introduced new violet dyes: BD Horizon V450, V500, and V550.

Purpose of the Study:

• To evaluate the performance of new BD Horizon violet-excitable dyes (V450, V500, V550) for FCM.
• To assess the utility of these dyes in multicolor FCM applications.
• To compare the spectral properties and compatibility of the new dyes with existing reagents.

Main Methods:

• Development of functional antibody conjugates using BD Horizon V450, V500, and V550 dyes.
• Testing of these conjugates in multilaser and polychromatic flow cytometry.
• Comparison of performance against a panel of existing violet-excitable FCM reagents.
• Evaluation of V500 dye compatibility with FITC in multicolor panels.

Main Results:

• BD Horizon V450, V500, and V550 antibody conjugates demonstrate good performance within a useful sensitivity range.
• These dyes are compatible with multilaser flow cytometry for two- and three-color combinations.
• BD Horizon V500 offers improved spectral coverage, filling a gap in the market.
• V500 exhibits superior compatibility with FITC in multicolor applications compared to AmCyan.

Conclusions:

• The new BD Horizon violet-excitable dyes provide valuable reagents for multicolor flow cytometry.
• BD Horizon V500 is a particularly advantageous dye for multicolor panels due to its spectral properties and FITC compatibility.
• These advancements expand the capabilities of flow cytometry analysis.