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05:02
Listeria monocytogenes Infection of the Brain
Published on: October 2, 2018
VIDAS Listeria monocytogenes II (LMO2)
1bioMérieux, Inc., 595 Anglum Rd, Hazelwood, MO 63042, USA. ron.johnson@biomerieux.com
Journal of AOAC International
|June 18, 2013
Summary
The VIDAS Listeria monocytogenes II (LMO2) method is validated as an accurate alternative for detecting L. monocytogenes in ready-to-eat meats. This automated immunoassay showed no statistical difference compared to the MFHPB-30 reference method.
Area of Science:
- Food Safety and Microbiology
- Analytical Chemistry
- Diagnostic Assays
Background:
- Listeria monocytogenes is a significant foodborne pathogen.
- Accurate detection in ready-to-eat (RTE) meats is crucial for public health.
- Existing reference methods require validation with newer technologies.
Purpose of the Study:
- To compare the performance of the VIDAS Listeria monocytogenes II (LMO2) assay against the MFHPB-30 reference method.
- To evaluate the efficacy of chromogenic media for confirming LMO2 presumptive results.
- To establish LMO2 as an acceptable alternative method for L. monocytogenes detection in RTE meats.
Main Methods:
- AOAC Government Validation (GovVal) study design.
- Comparative analysis of VIDAS LMO2 (automated immunoassay) and MFHPB-30 (culture-based).
- Use of chromogenic media (chromID Ottaviani Agosti Agar and chromID Lmono) for confirmation.
Main Results:
- No statistically significant differences were observed between VIDAS LMO2 and MFHPB-30 methods (p < 0.05).
- Chromogenic media confirmed presumptive LMO2 results comparably to reference agars.
- LMO2 demonstrated acceptable performance across various RTE meat matrices.
Conclusions:
- The VIDAS LMO2 method is a reliable and acceptable alternative for detecting L. monocytogenes in RTE meats.
- The study supports the use of LMO2 in routine food safety testing.
- Chromogenic media effectively aids in the confirmation step of the LMO2 assay.
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