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Updated: May 10, 2026

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
10:58

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue

Published on: October 1, 2008

Neuron enriched nuclear proteome isolated from human brain.

Eric B Dammer1, Duc M Duong, Ian Diner

  • 1Department of Human Genetics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

Journal of Proteome Research
|June 18, 2013
PubMed
Summary
This summary is machine-generated.

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Researchers isolated neuronal and non-neuronal nuclei from human brain tissue using fluorescence-activated nuclei sorting (FANS). Proteomics identified cell-type-specific proteins, revealing differences in neurons and astrocytes, crucial for understanding brain aging and neurodegenerative diseases.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Proteomics

Background:

  • The human brain comprises diverse cell types, including neurons, astrocytes, oligodendrocytes, and microglia.
  • Understanding cell-type-specific nuclear components is vital for studying brain aging and neurodegenerative diseases.

Purpose of the Study:

  • To isolate and proteomically characterize neuronal and non-neuronal nuclei from human brain tissue.
  • To identify cell-type-specific nuclear proteins and their alterations in aging or disease.

Main Methods:

  • Utilized fluorescence-activated nuclei sorting (FANS) to purify intact neuronal (NeuN-positive) and non-neuronal nuclei from post-mortem human brain.
  • Employed mass spectrometry (MS) based label-free quantitative proteomics to identify and quantify nuclear proteins.

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Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

Published on: April 16, 2021

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Last Updated: May 10, 2026

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
10:58

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue

Published on: October 1, 2008

Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
08:18

Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting

Published on: April 16, 2021

  • Validated differential protein enrichment using immunoblots on primary cultured cells.

    • Main Results:

    • Identified 1755 proteins from sorted neuronal and non-neuronal nuclear extracts.
    • Approximately 20% of identified proteins showed significant enrichment or depletion in neuronal versus non-neuronal populations.
    • Confirmed cell-type-specific enrichment of nesprin-1 and nesprin-3 within the LINC complex in neurons and astrocytes, respectively.
    • Discovered several transcription and splicing factors with cell-type-specific enrichment in the human brain.

    Conclusions:

    • Fluorescence-activated nuclei sorting (FANS) coupled with quantitative proteomics is effective for cell-type-specific nuclear proteome analysis in the human brain.
    • The study identified novel cell-type-specific nuclear proteins, including components of the LINC complex and regulatory factors.
    • These findings provide a foundation for understanding cell-type-specific nuclear changes in brain aging and neurodegeneration.