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Updated: May 10, 2026
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
Published on: June 14, 2020
Concentration of biologically active compounds extracted from Ilex paraguariensis St. Hil. by nanofiltration
Aureanna Nairne Negrão Murakami1, Renata Dias de Mello Castanho Amboni, Elane Schwinden Prudêncio
1Department of Food Science and Technology, Federal University of Santa Catarina, Rodovia Admar Gonzaga, 1346, 88034-001 Florianópolis, SC, Brazil.
Abstract:
The aim of this study was to characterise the bioactive compounds in mate (Ilex paraguariensis St. Hil) extract and in concentrated mate extract obtained by nanofiltration (NF). Also, the impact of NF on the antioxidant activity of both mate extracts was evaluated in vitro and using eukaryotic cells of Saccharomyces cerevisiae (yeast assay). The results showed a significant increase in the contents of total phenolics (338%), chlorogenic acid (483%), theobromine (323%), caffeine (251%), chlorophyll (321%), condensed tannins (278%) and saponins (211%) in the concentrated mate extract. The concentrated mate extract showed higher in vitro antioxidant activity than the mate extract. According to the results obtained, it can be stated that the use of nanofiltration membrane is a valid approach for the concentration of biologically active compounds in aqueous extract of mate.
