Related Experiment Video
Updated: May 10, 2026
Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves
Published on: June 15, 2019
Label-free impedimetric immunosensor for sensitive detection of fenvalerate in tea
Meirong Wang1, Huimin Kang, Dan Xu
1College of Chemistry and Chemical Engineering, Jiangsu Key Laboratory of Environmental Engineering and Monitoring, Yangzhou University, 180 Si-Wang-Ting Road, Yangzhou 225002, China.
Abstract:
In this experiment, fenvalerate antibodies were immobilised on the electrode by the crosslinking with glutaraldehyde modified on the glassy carbon electrode (GCE) via chitosan. Fenvalerate was measured by the increase of electron transfer resistance when the immune reaction occurred with Fe(CN)6(3-)/Fe(CN)6(4-) as the probe. Under optimal conditions, the change of resistance is in a linear relationship with the logarithm of the concentration in the range of 1.0×10(-3)∼1.0×10(1)mg/L (R=0.998) with a detection limit of 0.80 μg/L. This method bears such merits as simplicity of operation, high sensitivity, wide linear range, specificity, reproducibility and good stability. The immunosensor was applied in the detection of real samples of tea, achieving satisfactory results, and it could be regenerated after being placed alternately in 0.5 mol/L HCl and 0.5 mol/L NaOH solutions.
