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Updated: May 10, 2026
A Murine Model of Carotid Aneurysm Formation
Published on: September 9, 2025
Correlation between MMP-2 and NF-κ B expression of intracranial aneurysm
1Department of Neurosurgery, Xuanwu Capital University, Beijing 100053, China.
Objective:
To investigate the correlation between expressions of MMP-2 and NF-κ B in the intracranial aneurysm wall, and explore their role in the mechanism of the occurrence, growth and rupture of intracranial aneurysms.
Methods:
RT-PCR was used to detect the expression of MMP-2 and NF-κ B mRNA of 30 cases of intracranial aneurysm tissue and 10 cases of normal intracranial arterial tissue; Immunohistochemical method was used to detect the expression of MMP-2 and NF-κ B protein.
Results:
The semi-quantitative analysis of MMP-2 and NF-κ B in aneurysms tissues and normal tissues were statistically significant different from each other (P<0 05). Immunohistochemical staining results showed NF-κ B was expressed in different layers. The expression of them were positive in intimal and medial, and the expression sites were located in the nucleus. MMP-2 were expressed in different layers of the aneurysm wall, and the expressions were positive in media and extima. The MMP-2 and NF- κ B-positive expression of aneurysm wall were significantly higher than in normal cerebral arteries (P <0.05). MMP-2 and NF-κ B mRNA expression showed positive correlation in the aneurysm wall tissue (r = 0.689, P = 0.005).
Conclusions:
The expressions of MMP-2 and NF-κ B in the intracranial aneurysm wall tissue were significantly higher than in the normal intracranial arterial tissues. They have a synergistic effect on the formation of intracranial aneurysms.
Insights
Matrix metalloproteinase-2 (MMP-2) and Nuclear factor-kappa B (NF-κB) are elevated in intracranial aneurysm walls. These proteins synergistically contribute to aneurysm formation, growth, and rupture.
Area of Science:
- Neuroscience
- Molecular Biology
- Pathology
Background:
- Intracranial aneurysms (IAs) are cerebrovascular abnormalities with significant morbidity and mortality.
- The molecular mechanisms underlying IA formation, growth, and rupture remain incompletely understood.
- Identifying key molecular players is crucial for developing targeted therapeutic strategies.
Purpose of the Study:
- To investigate the correlation between matrix metalloproteinase-2 (MMP-2) and nuclear factor-kappa B (NF-κB) expression in intracranial aneurysm walls.
- To explore the role of MMP-2 and NF-κB in the pathogenesis of intracranial aneurysms.
Main Methods:
- Real-time quantitative polymerase chain reaction (RT-PCR) was employed to assess MMP-2 and NF-κB mRNA levels in 30 IA tissues and 10 normal arterial tissues.
- Immunohistochemical analysis was performed to detect the protein expression and localization of MMP-2 and NF-κB within the aneurysm wall.
Main Results:
- MMP-2 and NF-κB expression (mRNA and protein) were significantly higher in IA tissues compared to normal arterial tissues (P < 0.05).
- NF-κB was localized in the nucleus of intimal and medial cells, while MMP-2 was found in the media and extima of the aneurysm wall.
- A significant positive correlation was observed between MMP-2 and NF-κB mRNA expression in IA tissues (r = 0.689, P = 0.005).
Conclusions:
- Elevated expression of MMP-2 and NF-κB in intracranial aneurysm walls suggests their involvement in IA pathogenesis.
- MMP-2 and NF-κB exhibit a synergistic effect, contributing to the occurrence, growth, and potential rupture of intracranial aneurysms.
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