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Amyloid Fibrils03:03

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Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
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Proteins behaving badly: emerging technologies in profiling biopharmaceutical aggregation.

Zahra Hamrang1, Nicholas J W Rattray, Alain Pluen

  • 1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Manchester, UK.

Trends in Biotechnology
|June 18, 2013
PubMed
Summary

Predicting protein aggregation in biopharmaceuticals is challenging due to analytical method artifacts. Emerging technologies offer promising solutions for accurate aggregation prediction and profiling in biopharmaceutical development.

Keywords:
aggregationbiopharmaceuticalsmanufactureproduct developmentquality by design

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Area of Science:

  • Biotechnology and biopharmaceutical development.
  • Biochemistry and biophysics.
  • Analytical chemistry and protein science.

Background:

  • Biotechnology advances, driven by biochemical and biophysical instrumentation, have enabled high-throughput screening, recombinant DNA technology, and monoclonal antibody production.
  • These innovations have solidified the biopharmaceutical industry's position in the therapeutic market.
  • However, accurately predicting and profiling protein aggregation during biopharmaceutical formulation remains a significant challenge due to method-specific artifacts.

Purpose of the Study:

  • To review emerging trends and novel technologies for protein aggregation prediction.
  • To highlight methods for accurate profiling of protein aggregation throughout biopharmaceutical product design.

Main Methods:

  • Review of current scientific literature on protein aggregation analysis.
  • Analysis of emerging trends and novel technologies in biophysical and biochemical instrumentation.
  • Evaluation of methods for predicting and profiling protein aggregation.

Main Results:

  • Existing analytical methods present artifacts that complicate accurate protein aggregation prediction.
  • Novel technologies and emerging trends show potential for improved aggregation assessment.
  • These advancements may enable more reliable prediction and profiling of protein aggregation.

Conclusions:

  • Accurate prediction and profiling of protein aggregation are critical for successful biopharmaceutical formulation.
  • Emerging technologies offer promising avenues to overcome current analytical limitations.
  • Further research and adoption of these novel methods are essential for advancing biopharmaceutical product development.